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Complete sequence of a mink astrovirus (March 2003)

The complete genome sequence of an astrovirus of mink has been released on the databases [AY179509].

Avian nephritis virus 2 (ANV-2)

The sequence of ORF 2 (capsid-coding region) of avian nephritis virus 2 has been released on the EMBL/GenBank/DDBJ databases. Like ANV-1 it is an astrovirus (see below).

Avian nephritis virus 1 (ANV-1)

The complete genome sequence of avian nephritis virus 1 (ANV-1, strain G-4260) has been deposited on the EMBL/GenBank/DDBJ databases (accession no. AB033998). This sequence shows it to be a member of the Astroviridae and not a picornavirus as it is currently classified.

Imada, T., Yamaguchi, S., Mase, M., Tsukamoto, K., Kubo, M., Morooka, A. (2000). Avian nephritis virus (ANV) as a new member of the family Astroviridae and construction of infectious ANV cDNA. J. Virol. 74: 8487-8493.

Tadao Imada, National Institute of Animal Health, Department of Virology, 3-1-1, Kannondai, Tsukuba, Ibaraki 305-0856, Japan. (E-mail: imadatad@niah.affrc.go.jp, URL: http://ss.niah.affrc.go.jp/NIAH/hoge-s.html, Tel: 81-298-38-7760, Fax:81-298-38-7760)

Abstract: We have constructed a genome-length cDNA clone for human astrovirus serotype 1. When a human colon cancer-derived cell line, CaCo-2, is transfected with RNA transcribed in vitro from this cDNA clone, infectious virus is produced at titers close to those observed after infection with intact astrovirus. A rodent cell line, BHK, which is largely refractory to astrovirus infection, was found to support efficient growth of the virus if transfected with viral RNA. The high transfection efficiency seen in the BHK cells allows studies of the viral replication in the transfected cells and thus should prove useful for the characterization of noninfectious astroviral mutants.